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alpha mouse liver 12 aml12 mouse hepatocyte cell line  (ATCC)


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    ATCC alpha mouse liver 12 aml12 mouse hepatocyte cell line
    Alpha Mouse Liver 12 Aml12 Mouse Hepatocyte Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1683 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    alpha mouse liver 12 aml12 mouse hepatocyte cell line  (ATCC)
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    alpha mouse liver 12 aml12 hepatocyte cell line  (ATCC)
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    ATCC alpha mouse liver 12 aml12 hepatocyte cell line
    (A) A western blot analysis of lysates from <t>AML12</t> mouse hepatocytes challenged with Smoothened agonist (SAG) for the indicated times. Insulin treatment is a positive control for AKT phosphorylation. (B–G) Quantification of western blot images for the indicated phosphoproteins relative to their total protein levels (mean ± SEM; * p < 0.05; ** p < 0.01; n.s., not significant; t test). The GLI1 protein is normalized to actin levels. Either the 10- or 15-min time point was used for quantification (see ). (H) A western blot analysis of lysates from cells transfected with either mock or Rictor siRNAs and challenged with vehicle, 0.5 μM SAG (15 min), or 100 nM insulin (30 min). (I) DEGs in the rict-1(mg360) mutant compared to the grd triple mutant (hypergeometric p value reported). (J) A scatterplot showing the differential expression values for the rict-1(mg360) mutant plotted against the grd mutant ( rict-1 -specific DEGs in red, grd -specifc DEGs in blue, and rict-1/grd co-regulated genes in black). R 2 values are reported for linear regression analyses on the union and intersection of the rict-1 and grd DEG datasets. (K and L) (K) Representative images (scale bar, 50 mm; white arrowheads indicate nuclei) and (L) quantification of DAF-16::mKate2 and GFP::PQM-1 nuclear fluorescence in day 1 adult WT and grd mutant animals reared at 20°C (mean ± SD; **** p < 0.0001; t test). (M) The average DAF-16 (left) or PQM-1 (right) enrichment (ChIP-seq signal) at the transcriptional start site (TSS) at all genes (red) or at genes differentially expressed in the grd mutant (black). See also .
    Alpha Mouse Liver 12 Aml12 Hepatocyte Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alpha mouse liver 12 aml12 hepatocyte cell line/product/ATCC
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    mouse hepatocyte cell line alpha mouse liver aml  (ATCC)
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    ATCC mouse hepatocyte cell line alpha mouse liver aml
    (A) A western blot analysis of lysates from <t>AML12</t> mouse hepatocytes challenged with Smoothened agonist (SAG) for the indicated times. Insulin treatment is a positive control for AKT phosphorylation. (B–G) Quantification of western blot images for the indicated phosphoproteins relative to their total protein levels (mean ± SEM; * p < 0.05; ** p < 0.01; n.s., not significant; t test). The GLI1 protein is normalized to actin levels. Either the 10- or 15-min time point was used for quantification (see ). (H) A western blot analysis of lysates from cells transfected with either mock or Rictor siRNAs and challenged with vehicle, 0.5 μM SAG (15 min), or 100 nM insulin (30 min). (I) DEGs in the rict-1(mg360) mutant compared to the grd triple mutant (hypergeometric p value reported). (J) A scatterplot showing the differential expression values for the rict-1(mg360) mutant plotted against the grd mutant ( rict-1 -specific DEGs in red, grd -specifc DEGs in blue, and rict-1/grd co-regulated genes in black). R 2 values are reported for linear regression analyses on the union and intersection of the rict-1 and grd DEG datasets. (K and L) (K) Representative images (scale bar, 50 mm; white arrowheads indicate nuclei) and (L) quantification of DAF-16::mKate2 and GFP::PQM-1 nuclear fluorescence in day 1 adult WT and grd mutant animals reared at 20°C (mean ± SD; **** p < 0.0001; t test). (M) The average DAF-16 (left) or PQM-1 (right) enrichment (ChIP-seq signal) at the transcriptional start site (TSS) at all genes (red) or at genes differentially expressed in the grd mutant (black). See also .
    Mouse Hepatocyte Cell Line Alpha Mouse Liver Aml, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse hepatocyte cell line alpha mouse liver 12  (ATCC)
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    ATCC mouse hepatocyte cell line alpha mouse liver 12
    (A) A western blot analysis of lysates from <t>AML12</t> mouse hepatocytes challenged with Smoothened agonist (SAG) for the indicated times. Insulin treatment is a positive control for AKT phosphorylation. (B–G) Quantification of western blot images for the indicated phosphoproteins relative to their total protein levels (mean ± SEM; * p < 0.05; ** p < 0.01; n.s., not significant; t test). The GLI1 protein is normalized to actin levels. Either the 10- or 15-min time point was used for quantification (see ). (H) A western blot analysis of lysates from cells transfected with either mock or Rictor siRNAs and challenged with vehicle, 0.5 μM SAG (15 min), or 100 nM insulin (30 min). (I) DEGs in the rict-1(mg360) mutant compared to the grd triple mutant (hypergeometric p value reported). (J) A scatterplot showing the differential expression values for the rict-1(mg360) mutant plotted against the grd mutant ( rict-1 -specific DEGs in red, grd -specifc DEGs in blue, and rict-1/grd co-regulated genes in black). R 2 values are reported for linear regression analyses on the union and intersection of the rict-1 and grd DEG datasets. (K and L) (K) Representative images (scale bar, 50 mm; white arrowheads indicate nuclei) and (L) quantification of DAF-16::mKate2 and GFP::PQM-1 nuclear fluorescence in day 1 adult WT and grd mutant animals reared at 20°C (mean ± SD; **** p < 0.0001; t test). (M) The average DAF-16 (left) or PQM-1 (right) enrichment (ChIP-seq signal) at the transcriptional start site (TSS) at all genes (red) or at genes differentially expressed in the grd mutant (black). See also .
    Mouse Hepatocyte Cell Line Alpha Mouse Liver 12, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    non hepatoma hepatocyte cell line alpha mouse liver aml 12  (ATCC)
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    ATCC non hepatoma hepatocyte cell line alpha mouse liver aml 12
    (A) A western blot analysis of lysates from <t>AML12</t> mouse hepatocytes challenged with Smoothened agonist (SAG) for the indicated times. Insulin treatment is a positive control for AKT phosphorylation. (B–G) Quantification of western blot images for the indicated phosphoproteins relative to their total protein levels (mean ± SEM; * p < 0.05; ** p < 0.01; n.s., not significant; t test). The GLI1 protein is normalized to actin levels. Either the 10- or 15-min time point was used for quantification (see ). (H) A western blot analysis of lysates from cells transfected with either mock or Rictor siRNAs and challenged with vehicle, 0.5 μM SAG (15 min), or 100 nM insulin (30 min). (I) DEGs in the rict-1(mg360) mutant compared to the grd triple mutant (hypergeometric p value reported). (J) A scatterplot showing the differential expression values for the rict-1(mg360) mutant plotted against the grd mutant ( rict-1 -specific DEGs in red, grd -specifc DEGs in blue, and rict-1/grd co-regulated genes in black). R 2 values are reported for linear regression analyses on the union and intersection of the rict-1 and grd DEG datasets. (K and L) (K) Representative images (scale bar, 50 mm; white arrowheads indicate nuclei) and (L) quantification of DAF-16::mKate2 and GFP::PQM-1 nuclear fluorescence in day 1 adult WT and grd mutant animals reared at 20°C (mean ± SD; **** p < 0.0001; t test). (M) The average DAF-16 (left) or PQM-1 (right) enrichment (ChIP-seq signal) at the transcriptional start site (TSS) at all genes (red) or at genes differentially expressed in the grd mutant (black). See also .
    Non Hepatoma Hepatocyte Cell Line Alpha Mouse Liver Aml 12, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    non transformed mouse hepatocyte cell line alpha mouse liver 12  (ATCC)
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    ATCC non transformed mouse hepatocyte cell line alpha mouse liver 12
    (A) A western blot analysis of lysates from <t>AML12</t> mouse hepatocytes challenged with Smoothened agonist (SAG) for the indicated times. Insulin treatment is a positive control for AKT phosphorylation. (B–G) Quantification of western blot images for the indicated phosphoproteins relative to their total protein levels (mean ± SEM; * p < 0.05; ** p < 0.01; n.s., not significant; t test). The GLI1 protein is normalized to actin levels. Either the 10- or 15-min time point was used for quantification (see ). (H) A western blot analysis of lysates from cells transfected with either mock or Rictor siRNAs and challenged with vehicle, 0.5 μM SAG (15 min), or 100 nM insulin (30 min). (I) DEGs in the rict-1(mg360) mutant compared to the grd triple mutant (hypergeometric p value reported). (J) A scatterplot showing the differential expression values for the rict-1(mg360) mutant plotted against the grd mutant ( rict-1 -specific DEGs in red, grd -specifc DEGs in blue, and rict-1/grd co-regulated genes in black). R 2 values are reported for linear regression analyses on the union and intersection of the rict-1 and grd DEG datasets. (K and L) (K) Representative images (scale bar, 50 mm; white arrowheads indicate nuclei) and (L) quantification of DAF-16::mKate2 and GFP::PQM-1 nuclear fluorescence in day 1 adult WT and grd mutant animals reared at 20°C (mean ± SD; **** p < 0.0001; t test). (M) The average DAF-16 (left) or PQM-1 (right) enrichment (ChIP-seq signal) at the transcriptional start site (TSS) at all genes (red) or at genes differentially expressed in the grd mutant (black). See also .
    Non Transformed Mouse Hepatocyte Cell Line Alpha Mouse Liver 12, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non transformed mouse hepatocyte cell line alpha mouse liver 12/product/ATCC
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    Image Search Results


    (A) A western blot analysis of lysates from AML12 mouse hepatocytes challenged with Smoothened agonist (SAG) for the indicated times. Insulin treatment is a positive control for AKT phosphorylation. (B–G) Quantification of western blot images for the indicated phosphoproteins relative to their total protein levels (mean ± SEM; * p < 0.05; ** p < 0.01; n.s., not significant; t test). The GLI1 protein is normalized to actin levels. Either the 10- or 15-min time point was used for quantification (see ). (H) A western blot analysis of lysates from cells transfected with either mock or Rictor siRNAs and challenged with vehicle, 0.5 μM SAG (15 min), or 100 nM insulin (30 min). (I) DEGs in the rict-1(mg360) mutant compared to the grd triple mutant (hypergeometric p value reported). (J) A scatterplot showing the differential expression values for the rict-1(mg360) mutant plotted against the grd mutant ( rict-1 -specific DEGs in red, grd -specifc DEGs in blue, and rict-1/grd co-regulated genes in black). R 2 values are reported for linear regression analyses on the union and intersection of the rict-1 and grd DEG datasets. (K and L) (K) Representative images (scale bar, 50 mm; white arrowheads indicate nuclei) and (L) quantification of DAF-16::mKate2 and GFP::PQM-1 nuclear fluorescence in day 1 adult WT and grd mutant animals reared at 20°C (mean ± SD; **** p < 0.0001; t test). (M) The average DAF-16 (left) or PQM-1 (right) enrichment (ChIP-seq signal) at the transcriptional start site (TSS) at all genes (red) or at genes differentially expressed in the grd mutant (black). See also .

    Journal: Cell reports

    Article Title: Non-cell-autonomous regulation of mTORC2 by Hedgehog signaling maintains lipid homeostasis

    doi: 10.1016/j.celrep.2024.115191

    Figure Lengend Snippet: (A) A western blot analysis of lysates from AML12 mouse hepatocytes challenged with Smoothened agonist (SAG) for the indicated times. Insulin treatment is a positive control for AKT phosphorylation. (B–G) Quantification of western blot images for the indicated phosphoproteins relative to their total protein levels (mean ± SEM; * p < 0.05; ** p < 0.01; n.s., not significant; t test). The GLI1 protein is normalized to actin levels. Either the 10- or 15-min time point was used for quantification (see ). (H) A western blot analysis of lysates from cells transfected with either mock or Rictor siRNAs and challenged with vehicle, 0.5 μM SAG (15 min), or 100 nM insulin (30 min). (I) DEGs in the rict-1(mg360) mutant compared to the grd triple mutant (hypergeometric p value reported). (J) A scatterplot showing the differential expression values for the rict-1(mg360) mutant plotted against the grd mutant ( rict-1 -specific DEGs in red, grd -specifc DEGs in blue, and rict-1/grd co-regulated genes in black). R 2 values are reported for linear regression analyses on the union and intersection of the rict-1 and grd DEG datasets. (K and L) (K) Representative images (scale bar, 50 mm; white arrowheads indicate nuclei) and (L) quantification of DAF-16::mKate2 and GFP::PQM-1 nuclear fluorescence in day 1 adult WT and grd mutant animals reared at 20°C (mean ± SD; **** p < 0.0001; t test). (M) The average DAF-16 (left) or PQM-1 (right) enrichment (ChIP-seq signal) at the transcriptional start site (TSS) at all genes (red) or at genes differentially expressed in the grd mutant (black). See also .

    Article Snippet: The alpha mouse liver 12 (AML12) hepatocyte cell line was isolated from a 3-month-old male mouse liver (CD1 strain, line MT42) transgenic for human TGFα (CRL-2254, ATCC).

    Techniques: Western Blot, Positive Control, Phospho-proteomics, Transfection, Mutagenesis, Quantitative Proteomics, Fluorescence, ChIP-sequencing

    (A) The rict-1/grd co-regulated genes are enriched for pmk-1 -dependent genes (hypergeometric p value reported ). (B) Quantification of lipid levels using Nile Red staining (day 1 adults reared at 20°C; mean ± SD; ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA). (C) A heatmap showing differential expression values (log2 fold change relative to WT) of the 1,632 grd -dependent genes ( R 2 values are shown for the indicated comparisons). (D–G) Western blot and quantification of phospho-p38/PMK-1 levels for (D and E) C. elegans lysates prepared from WT, rict-1(mg360) , grd , or nsy-1(ums8) mutants ( ums8 is a gain-of-function allele; mean ± SEM; * p < 0.05; n.s., not significant; one-way ANOVA) and (F and G) AML12 hepatocytes treated with SAG for increasing amounts of time (10- or 15-min time point quantified; mean ± SEM; ** p < 0.01; t test). (H) A model of how Hh governs intestinal metabolism through the dual regulation of mTORC2 and p38 signaling in C. elegans . See also .

    Journal: Cell reports

    Article Title: Non-cell-autonomous regulation of mTORC2 by Hedgehog signaling maintains lipid homeostasis

    doi: 10.1016/j.celrep.2024.115191

    Figure Lengend Snippet: (A) The rict-1/grd co-regulated genes are enriched for pmk-1 -dependent genes (hypergeometric p value reported ). (B) Quantification of lipid levels using Nile Red staining (day 1 adults reared at 20°C; mean ± SD; ** p < 0.01; *** p < 0.001; **** p < 0.0001; one-way ANOVA). (C) A heatmap showing differential expression values (log2 fold change relative to WT) of the 1,632 grd -dependent genes ( R 2 values are shown for the indicated comparisons). (D–G) Western blot and quantification of phospho-p38/PMK-1 levels for (D and E) C. elegans lysates prepared from WT, rict-1(mg360) , grd , or nsy-1(ums8) mutants ( ums8 is a gain-of-function allele; mean ± SEM; * p < 0.05; n.s., not significant; one-way ANOVA) and (F and G) AML12 hepatocytes treated with SAG for increasing amounts of time (10- or 15-min time point quantified; mean ± SEM; ** p < 0.01; t test). (H) A model of how Hh governs intestinal metabolism through the dual regulation of mTORC2 and p38 signaling in C. elegans . See also .

    Article Snippet: The alpha mouse liver 12 (AML12) hepatocyte cell line was isolated from a 3-month-old male mouse liver (CD1 strain, line MT42) transgenic for human TGFα (CRL-2254, ATCC).

    Techniques: Staining, Quantitative Proteomics, Western Blot

    key resources table

    Journal: Cell reports

    Article Title: Non-cell-autonomous regulation of mTORC2 by Hedgehog signaling maintains lipid homeostasis

    doi: 10.1016/j.celrep.2024.115191

    Figure Lengend Snippet: key resources table

    Article Snippet: The alpha mouse liver 12 (AML12) hepatocyte cell line was isolated from a 3-month-old male mouse liver (CD1 strain, line MT42) transgenic for human TGFα (CRL-2254, ATCC).

    Techniques: Virus, Recombinant, SYBR Green Assay, Transfection, DC Protein Assay, Mutagenesis, CRISPR, Plasmid Preparation, Software